0README

|-- enzyme-efficiency
|   |-- enz-effic.mm
|   `-- enz-effic.mm.ps



|-- enzyme-efficiency

|   |-- enz-effic.mm
|   |-- enz-effic.mm.ps
	Why are enzymes so effective as catalysts?
	Why is all that protein there?
    	4 pages
	outline form
	troff and PS files;
		I. What factors or processes account for the high
		efficiency of enzyme catalysis.
			1. REDUCTION OF CHARGE SEPARATION IN TS
				ELECTROSTATIC CATALYSIS
			2. REDUCTION OF $T DELTA S*"$
			3. APPROXIMATION AND ORIENTATION
			4. STRAIN AND STRESS
			5. ENZYME BINDS TS (COMPLEMENTARY TO TS)
			6. EVOLUTION
			7. ACTIVE SITE "SOLVATES" REACTANT AND CATALYTIC GROUPS
				REDUCTION OF SOLVENT REORGANIZATION ENERGY
				(also 1 above)
			8. LOW BARRIER HYDROGEN BONDS
				(1 and 4 above)
			9. CONTROL OF REACTION PATH
			10. SEQUENCE OF ES INTERMEDIATES
			11. DYNAMICS
		II. What are the special properties of proteins
		that contribute to the high efficiency of enzyme
		catalysis? [ Or, why is all that protein there?]
			1. SEQUENCE OF ES INTERMEDIATES
			2. IMPORTANCE OF LONG-LIFETIME INTERMEDIATES
			3. EVOLUTION
			4. ORGANIZATION OF THE ACTIVE SITE 
				(1, 2, 3, and 7 above)
			5. CONTROL OF REACTION PATH
	9


John Rupley
 rupley@u.arizona.edu -or- jar@rupley.com
 30 Calle Belleza, Tucson AZ 85716 - (520) 325-4533; fax - (520) 325-4991
 Dept. Biochemistry & Molecular Biophysics, Univ. Arizona, Tucson AZ 85721