0README

enzyme-kinetics-MM
`-- 0README                 obvious


It is essentially trivial to write an R-language function to fit
the Michaelis-Menten model to enzyme kinetic data.  

But why do the work, when then R group has done it for you, and
done it well.  The current R distribution has a data set of enzyme
kinetic data (bring into frame with data(Puromycin)) and a built-in
function, "SSmicmen", for the Michaelis-Menten model. For suggestions
on carrying out an MM fit and plotting the results, see ?Puromycin
and ?SSmicmen


Another set of trial data: the enzyme-catalyzed reaction rate, "v",
in reciprocal seconds, as a function of the substrate concentration,
["S"], in mol/l, from kinetic measurements on lysozyme, which is
known to follow the Michaelis-Menten model.  The values of the
dependent variable, "v", have equal weights.

	   S              v

	.00015 M	.105  sec^-1
	.00009		.100
	.00006		.095
	.00006		.083
	.00004		.080
	.00004		.074
	.00003		.061
	.00002		.054
	.00002		.051



John Rupley
 rupley@u.arizona.edu -or- jar@rupley.com
 30 Calle Belleza, Tucson AZ 85716 - (520) 325-4533; fax - (520) 325-4991
 Dept. Biochemistry & Molecular Biophysics, Univ. Arizona, Tucson AZ 85721